2020/12/21 12:03:21
高通量組織研磨儀對真菌細胞的破壁及DNA提取
Source：Shanghai Jingxin
實驗?zāi)康?/strong>
對真菌細胞進行破壁，提取其DNA。
實驗原理
隨著分子生物學(xué)技術(shù)的進展，PCR相關(guān)方法越來越多地被應(yīng)用到真菌學(xué)研究中來，而真菌DNA的提取是包括分子診斷在內(nèi)的所有實驗的基礎(chǔ)，簡便快捷地提取真菌DNA，且獲得的DNA完整、純度高，對真菌分子生物學(xué)的研究有及其重要的意義。
真菌細胞壁以幾丁質(zhì)、葡萄糖為主構(gòu)成細胞壁骨架，而基質(zhì)則由多糖、甘露聚糖、糖蛋白、脂質(zhì)等填充。絲狀真菌堅韌厚壁的構(gòu)成，需要較為繁瑣的破壁手段，故真菌DNA的提取較細菌或其他組織細胞難度更大。為保證真菌PCR技術(shù)及其相關(guān)分子生物學(xué)技術(shù)的順利開展，簡便快捷地提取真菌DNA，且獲得的DNA完整、純度高，是必須解決的前提條件。
實驗材料和器具
樣品：真菌類
儀器：高通量組織研磨儀（上海凈信，Tissuelyser-48）
耗材：離心管，研磨珠（上海凈信）
試劑：TE Buffer，DA Buffer，E Binding Buffer，Elution Buffer
實驗步驟
需了解具體步驟，請致電廠家
實驗結(jié)果
圖1 提取DNA的檢測 M：100bp 標準參照物
圖2 提取DNA的PCR檢測結(jié)果 M：100bp 標準參照物
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